I've played it a few times and I have to agree with NerdinaNutshell that there are a few issues. It's still not clear to me why I'm taking damage. Are they attacking the location of the mouse? If so, it's not apparent. Before an attack, enemies should give you a clear warning. E.g a red flashing which gets quicker.
As for spawning, there's different ways of doing it. For example you could have a pupa appear which is harmless until an insect spawns from it. The graphics and UI need quite a bit of work, however, the game kept me interested enough to keep playing until I beat it, so you're doing something right. Some good gameplay is there.
About the boss level though, I must have reached it 6 or so times and it went on for ages (until I lost) but on the last run I beat it really quickly, and I don't know why.
I didn't get the Hudson Bee reference, though I have seen it before. Maybe parody it instead of hiding it.